Applications of a microplate reader in yeast physiology research
نویسندگان
چکیده
منابع مشابه
Applications of a microplate reader in yeast physiology research.
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed. The two main procedures involved are measuring growth curves and monitoring the pH changes of medium using two di...
متن کاملCounting nematodes with a microplate reader.
The feasibility of counting plant-parasitic nematodes in aqueous suspensions by measuring light transmittance through aqueous suspensions with an ELISA microplate reader was explored. Absorbance readings for eggs or vermiform stages of three species were linearly related (R(2) > 0.99) to concentrations between 0 and 10,000 nematodes/ml. Coefficients of variation ranged from 12-23%, depending on...
متن کاملRapid Measurement of Chlorophylls with a Microplate Reader
This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with cheap polystyrene plates; however, the principles apply t...
متن کاملUpgrading a microplate reader for photobiology and all-optical experiments.
Automation can vastly reduce the cost of experimental labor and thus facilitate high experimental throughput, but little off-the-shelf hardware for the automation of illumination experiments is commercially available. Here, we use inexpensive open-source electronics to add programmable illumination capabilities to a multimode microplate reader. We deploy this setup to characterize light-trigger...
متن کاملArtificial neural networks: applications in pain physiology
Artificial neural networks (ANNs) are intelligent systems that have successfully been used for prediction in different medical fields. In this study, the capability of ANN in predicting body behavior in pain-producing situations is evaluated. A three-layer back-propagation ANN is designed using MATLAB software. The inputs include the magnitude of stimulation in pain fibers, touch fibers and cen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2007
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112620